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bcl w  (Sino Biological)


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    Structured Review

    Sino Biological bcl w
    Bcl W, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcl+w/Human+BCL-W+%2F+BCL2L2+Protein/pmc12550731-377-44-52
    Average 93 stars, based on 1 article reviews
    bcl w - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Discovery of Dual BCL-xL/BCL-w Degraders by Exploiting the Bis(sulfonyl)benzene Ring of ABT-263 as a Linkage Vector
    Article Snippet: .. To a 96-well PCR plate (Thermo Scientific, Cat. No. AB-1400, MA, USA) were added 6 × His tagged protein [BCL-X L (10 uL, 1 nM, MilliporeSigma, Cat. No. SRP0187, MA, USA) or BCL-2 (10 uL, 2 nM, MilliporeSigma, Cat. No. SRP0186, MA, USA) or BCL-w (10 uL, 3 nM, Cat. No. 10059-H08E, SinoBiological, PA, USA)], testing compound (10 μ L, 3-fold serial dilution) and biotinylated peptide [BAD (10 uL, 2.5 nM, Biotin-LWAAQRYGRELRRMSDEFEGSFKGL) or BIM (10 uL, 20 nM for His-BCL-2 and 10 nM for His-BCL-w, Biotin-MRPEIWIAQELRRIGDEFNA), AnaSpec, CA, USA]. .. After the mixed solution was incubated at room temperature for 30 min, anti-6 × His acceptor beads (5 μ L, 160 μ g/mL, Cat. No. AL178M, Revvity, MA, USA) and streptavidin donor beads (5 μ L, 160 μ g/mL, Cat. No. 6760002, Revvity, MA, USA) were added to each well under subdued light and the mixture was incubated for an additional 30 min in the dark.

    Serial Dilution:

    Article Title: Discovery of Dual BCL-xL/BCL-w Degraders by Exploiting the Bis(sulfonyl)benzene Ring of ABT-263 as a Linkage Vector
    Article Snippet: .. To a 96-well PCR plate (Thermo Scientific, Cat. No. AB-1400, MA, USA) were added 6 × His tagged protein [BCL-X L (10 uL, 1 nM, MilliporeSigma, Cat. No. SRP0187, MA, USA) or BCL-2 (10 uL, 2 nM, MilliporeSigma, Cat. No. SRP0186, MA, USA) or BCL-w (10 uL, 3 nM, Cat. No. 10059-H08E, SinoBiological, PA, USA)], testing compound (10 μ L, 3-fold serial dilution) and biotinylated peptide [BAD (10 uL, 2.5 nM, Biotin-LWAAQRYGRELRRMSDEFEGSFKGL) or BIM (10 uL, 20 nM for His-BCL-2 and 10 nM for His-BCL-w, Biotin-MRPEIWIAQELRRIGDEFNA), AnaSpec, CA, USA]. .. After the mixed solution was incubated at room temperature for 30 min, anti-6 × His acceptor beads (5 μ L, 160 μ g/mL, Cat. No. AL178M, Revvity, MA, USA) and streptavidin donor beads (5 μ L, 160 μ g/mL, Cat. No. 6760002, Revvity, MA, USA) were added to each well under subdued light and the mixture was incubated for an additional 30 min in the dark.



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